Laser Assisted Hatching

  1. The laser assisted hatching is an additional lab technique designed to improve the efficiency of assisted reproductive technology procedures such as IVF & ICSI.
  2. The unfertilised egg is surrounded by a shell called zonapellucida The zona has an important role in fertilisation as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilisation.
  3. After fertilisation of the egg, during the cleavage stage of an embryo, zona pellucida hardens. This development is normal and the purpose is to keep the cells in the egg together.
  4. The embryos have to "hatch" or break out of the zona in order to embed into the endometrium lining of the uterine cavity. This occurs about 2-3 days after embryo transfer when the embryo is at the blastocyst stage. Naturally this takes place by expanding/contracting of the zona until it distorts, allowing the blastocyst to "hatch".
  5. When sperm and eggs are cultured in IVF laboratory, the zonapellucida hardens at much faster rate than natural, making it more difficult for the embryo to implant.
  6. Prior to implantation, the embryo has to escape out of its zonapellucida by a process known as hatching. If this process is not completed properly, implantation failure occurs and a pregnancy cannot be achieved.
  7. Assisted hatching is the process of creating a hole in zonapellucida, to aid the embryo in the hatching process.
  8. At IHR, we offer the Laser technology for Assisted Hatching (LAH), where a precision laser beam is focused over the zonapellucida making a small opening, between 10-20 microns to facilitate embryo hatching. LAH is done just before the Embryo Transfer.
  9. This helps to increase pregnancy rates by improving implantation rates, since embryo hatching is facilitated.
  10. A fast, safe, simple method compared to the old method of hatching which was performed by using acidic medium (expelled onto the zona to create a small hole).
  11. The accuracy of the laser is predetermined by its programming, and is therefore not dependent on the skill of the operator. There is a high degree of reproducible accuracy.
  12. We also recommend that thawed embryos should be treated with laser assisted hatching before Embryo Transfer to maximise success rates.

Who might benefit

  1. Patients with day 3 embryos having a thick Zona.
  2. Patients with failed previous IVF/ICSI cycles.
  3. Women older than 37 years of age, have a tendency to produce eggs with a harder zonapellucida than younger women.
  4. Women with a high Follicle Stimulating Hormone (FSH) level.
  5. Patients undergoing IVF/ICSI for the first time, who are considered poor responders because they have required a high dose of gonadotropins for poor ovarian response.
  6. Patients who in an earlier IVF cycle had low fertilisation rate, resulting in three or fewer embryos.
  7. Patients who request laser assisted hatching and are fully informed of its use and function.
  8. Patients who are under going frozen embryo transfer (FET).