Sperm Donor Selection Criteria

The Indian Council of Medical Research has instituted the following guidelines for Semen donor selection:

  1. Use of sperm donated by a relative or a known friend of either the wife or the husband shall not be permitted.
  2. Sperm donor should be between 21-45 years of age.
  3. The individual must be free of HIV and hepatitis B and C infections, hypertension, diabetes, sexually transmitted diseases, and identifiable and common genetic disorders such as thalassemia.
  4. An analysis must be carried out on the semen of the individual, preferably using a semen analyzer, and the semen must be found to be normal according to WHO method manual for semen analysis, if intended to be used for ART.
  5. The blood group and the Rh status of the individual must be determined and placed on record.
  6. Other relevant information in respect of the donor, such as height, weight, age, educational qualifications, profession, colour of the skin and the eyes, record of major diseases including any psychiatric disorder, and the family background in respect of history of any familial disorder, must be recorded in an appropriate proforma.
  7. Semen donor cannot be used for more than 10 successful pregnancies.
  8. Semen samples must be cryopreserved for at least six months before first use, at which time the semen donor must be tested for HIV and hepatitis B and C.

Sperm Donor Selection process:

Donor selection process is two step process which involves donor investigations and then donor registration.

Donor Investigations:

Semen donors need to undergo the following investigation within 15 days prior toregisteration:

  1. Blood group and Rh status
  2. Complete blood picture:
    1. Hb
    2. Total RBC count
    3. Total WBC count
    4. Differential WBC count
    5. Platelet count
    6. Peripheral smear
  3. Random blood sugar
  4. Blood urea / Serum creatinine
  5. SGPT
  6. Routine urine examination
  7. HBsAg status
  8. Hepatitis C status
  9. HIV (1) status with date of the tests done
  10. Hemoglobin A2 (for thallasemia) status
  11. HIV PCR (2) (positive or negative)
  12. Any other specific test (3)

  14. Height
  15. Weight
  16. Colour of skin
  17. Colour of hair
  18. Colour of eyes

  20. Pulse
  21. Blood pressure
  22. Temperature
  23. Respiratory system
  24. Cardiovascular system
  25. Per abdominal examination
  26. Other systems



(1) To be carried out every 6 months

(2) To be carried out if donor leaves within 6 months of the previous HIV test

(3) Any additional test carried out on the basis of the history and examination of donor

Donor Registeration:

If the investigations turn out to be normal, then they are made to fill up a Donor information form in which they are required to divulge the following information:


  1. Age / Date of birth
  2. Marital status
  3. Education :
    1. Donor
    2. Spouse
  4. Occupation :
    1. Donor
    2. Spouse
  5. Monthly income
  6. Religion

  8. Obstetric history of wife :
    1. Number of deliveries
    2. Number of abortions
    3. Other points of note
  9. History of use of contraceptives:

  10. Medical history
  11. Family history from the medical point of view
  12. History of any abnormality in a child of the donor
  13. History of blood transfusion
  14. History of substance abuse